1. What are the 5 stages of the cell cycle
2. How long is each phase in hours and what happens in each phase
3. Why is it essential that S and M phases alternate
4. What are the 4 cyclin Cdk complexes and what is the constitution of each
5. Which Cdk is essential for growth
6. What is the R point
7. How does cyclin D affect E2F and thus allow passage through the R point by transcription of genes that control entry into S phase
8. What is required at the checkpoint controlled by Cdk2
9. What are the 3 main CKIs
10. What is the role of Cdk7,8 and 9
11. What is a cyclin box
12. What is the difference between the Cip1/Kip1 and Ink4 CKI families and what are examples of each, which Cdks do they affect
13. In what 2 ways are cyclin levels regulated
14. What are the 2 ubiquitin ligase complexes involved in the cell cycle
15. What are the 4 cell cycle checkpoints and what happens when each is activated
16. How do Cdk4 and Cdk6 indirectly activate Cdk 2
17. What is DNA replication licensing and which phase does it occur in, what complex is formed
18. Which protein identifies and marks the origin as a landing pad
19. Which two proteins load the MCM4 helicase onto the protein above