1. What is the lumen of a vesicle topologically equivalent to
2. How can it be shown that targeting sequences are necessary and sufficient for protein targeting
3. Which receptor is mutated in Zellweger syndrome and what 3 amino acid sequence does it recognise
4. What do nuclear importins recognise
5. Which address label is required for mitochondrial targeting and which 2 membrane proteins facilitate the partial unfolding required
6. How does the type of targeting to the ER differ from that to other organelles
7. What is the ER targeting sequence
8. What binds to this and what consequences does binding have on translation
9. What protein is the receptor to the above protein attached to
10. Which terminus is on the cytosolic surface
11. What cleaves the recognition sequence in the lumen and which enzyme adds a branched chain of N linked asparagine glycosylation
12. Which two proteins on the ER membrane are associated with the ER associated degradation pathway
13. What is the most common CF mutation and how can it be shown that it is due to the ERAD pathway
14. Which glycoprotein modification is a signal for transport from the golgi to the lysosome via a late endosome
15. Which disease results from a lack of N-acetylglucosamine phosphotransferase
16. What is the function of rab proteins are when are they active
17. Which complex coats vesicles from ER to Golgi and which complex coats vesicles from Golgi to ER
18. What are the GM130 and p115 rabs
19. Which amino acid sequence is recognised by the receptor that COP I binds to