1. What is the equation for fraction bound given ligand concentration and Kd, what is the assumption here
2. Why is there great uncertainty in measuring high affinity interaction Kd
3. What is isothermal titration calorimetry
4. How does Kd relate to Gibbs free energy change
5. How can Kon and Koff be calculated by surface plasmon resonance
6. What type of receptor is in 30% of cancers
7. Which 3 domains are involved in EGFR ligand induced dimerization
8. What does dimerization lead to
9. Which SH2 protein binds the phosphotyrosine
10. How many AAs in the SH2 domain, What are the 3 secondary structures, Which 2 residues stabilise the phosphate
11. Why do SH2 domains have two binding pockets, what is the specificity sequence for the EGFR
12. What is the Gbr2 domain structure
13. How many amino acids is SH3 and what are its secondary structures
14. What does it bind with what buried area and which protein is this a part of
15. What type of protein is SOS
16. How does ras-GAP work
17. What forms between ras and Raf
18. Which domain of Raf forms this and thus how is the kinase activated
19. What is the cascade that ensues